ASTM D6499 (Inhibition ELISA)*
This assay uses an Inhibition ELISA to quantitate antigenic latex protein in product extracts. In an Inhibition ELISA, soluble antigens in the extract compete for binding to specific anti-latex antibodies and block antibody binding to latex antigen immobilized on the surface of the assay plate (the detection limit of the assay is 0.03 µg/ml).
ASTM D5712 Lowry with Precipitation*
Quantitatively measures the level of extractable proteins in latex products using a precipitation step to decrease the levels of interfering substances (5712 detection reporting limit of the assay is 9.7 µg/ml.).
Residual Glove Powder Analysis ASTM D6124
Quantitatively measures the amount of powder on the surface of gloves. The assay is performed by washing the glove with dH2O. The wash is then filtered through a glass fiber filter. The change in weight of the filter reflects the amount of powder present on the glove. Results are given in terms of mg powder/glove. The ASTM has set limits for the amount of powder on the surface of gloves. These standards are: <1mg/glove for "Powder Free" gloves, <10mg/glove fore Examination gloves, and <15mg/glove for Surgical gloves.
ASTM D7558 - Chemical Sensitivity
The chemical sensitivity assay was developed to quantify residual chemicals (vulcanization accelerators) used in the manufacturing of latex devises. Individuals can develop allergic contact dermatitis while being exposed to these residual chemicals. Monitoring the residual chemicals can give customers useful data to lower the amount of expose to these chemicals in their final products (the detection limit of the assay is 7.5 µg/ml).
* A2LA accredited assays.