The ASTM D6499 Assay is one of the most frequently performed tests at Guthrie.

* ASTM D6499-07 (Inhibition ELISA)
This assay uses an Inhibition ELISA to quantitate antigenic latex protein in product extracts. In an Inhibition ELISA, soluble antigens in the extract compete for binding to specific anti-latex antibodies and block antibody binding to latex antigen immobilized on the surface of the assay plate.

* ASTM D5712-05 Lowry with Precipitation
Quantitatively measures the level of extractable proteins in latex products using a precipitation step to decrease the levels of interfering substances (5712-05 reporting limit 42ug/gm).

The ASTM D7427-08 is a set of 4 ELISA tests for specific latex allergens. This assay uses monoclonal antibodies to quantitate the allergen levels

of Hev b1, Hev b3 and Hev b5 and Hev b6.02. This assay differs from the ASTM D6499 method in that it measures allergen content as apposed to antigenic content. The allergenic proteins are the ones that are recognized by IgE antibodies and elicit an allergic or anaphylactic response. This assay is very useful when you need to know the allergen level. The 6499 assay is used to quantitate all the latex proteins that elicit an antibody response. Because the 7427 assay uses monoclonal antibodies it is highly specific for the latex protein allergens. As the 6499 assay uses polyclonal sera there is the possibility that it may cross react with epitopes found on other proteins. (Please call or Email us to find out about availability and pricing.)

Western Blot Analysis
Qualitatively assesses the molecular weight distribution of antigenic protein in latex extracts by separating the proteins with SDS-PAGE, transferring them to a nitrocellulose or PVDF membrane and then identifying the latex proteins using anti-latex antibody.

Quantitatively measures the amount of powder on the surface of gloves. The assay is performed by washing the glove with dH2O. The wash is then filtered through a glass fiber filter. The change in weight of the filter reflects the amount of powder present on the glove. Results are given in terms of mg powder/glove. The ASTM has set limits for the amount of powder on the surface of gloves. These standards are: <1mg/glove for "Powder Free" gloves, <10mg/glove fore Examination gloves, and <15mg/glove for Surgical gloves.

ASTM D 7558-09 - Chemical Sensitivity

The chemical sensitivity assay was developed to quantify residual chemicals (vulcanization accelerators) used in the manufacturing of latex devises. Individuals can develop allergic contact dermatitis while being exposed to these residual chemicals. Monitoring the residual chemicals can give customers useful data to lower the amount of expose to these chemicals in their final products.

Guayule Latex ELISA

This assay uses an Inhibition ELISA to quantitate antigenic Guayule latex protein in product extracts. In an Inhibition ELISA, soluble antigens in the extract compete for binding to specific anti-latex antibodies and block antibody binding to latex antigen immobilized on the surface of the assay plate.

Latex in Air Analysis ELISA

This assay applies the D6499-07 assay in conjunction with air monitoring techniques. Suspect Latex contaminated air is filtered through air monitoring cassettes which is then is extracted from the filters and then analyzed utilizing the D6499-07 assay.

* - A2LA accredited assays.